Invited Paper BI+AS+BM+MS-WeM3
Development, Validation and Application of Q-Plex Array Technology
Wednesday, November 11, 2009, 8:40 am, Room K
The Quansys Q-Plex (multiplex ELISA) Array is a fully quantitative ELISA-based test where up to 25 distinct capture antibodies have been absorbed to each well of a 96-well plate in a defined array. This array is composed of 20 nanoliter spots with 350µm diameters and a pitch of 650µm between spots. Each spot represents a different distinct capture antibody population.
Using less than 30 µl of sample, up to 84 different samples can be assayed for all 25 unique analytes in less than 2.5 hours. Sensitivity is system dependent and typically ranges between 30 pg/ml to less than 1 pg/ml. All of the antibodies used in the Q-Plex arrays have been subject to a rigorous and comprehensive cross reactivity protocol and verified to be non-cross reactive with any other system on the array. Detection of this array is performed using the Quansys Q-View Imaging System. The image is then auto-processed using Quansys Q-View Software and concentrations for each analyte are output for the sample.