| AVS 55th International Symposium & Exhibition | |
| Synchrotron-based Spectroscopy and Spectro-Microscopy Topical Conference | Tuesday Sessions |
| Session SY+SS+BI-TuA |
| Session: | Synchrotron-based Spectroscopy and Spectro-Microscopy |
| Presenter: | A. Rosenhahn, University of Heidelberg, Germany |
| Authors: | A. Rosenhahn, University of Heidelberg, Germany R. Barth, University of Heidelberg, Germany F. Staier, University of Heidelberg, Germany C. Christoffis, University of Heidelberg, Germany T. Simpson, The University of Western Ontario, Canada S. Mittler, The University of Western Ontario, Canada S. Eisebitt, BESSY, Berlin, Germany M. Grunze, University of Heidelberg, Germany |
| Correspondent: | Click to Email |
Digital in-line soft X-ray holography (DIXH) is used as lenseless microscopy technique to investigate biological samples. The experimental setup follows directly the initial idea of Gabor,1 a holographic projection microscope based on a diverging photon beam. By creating a large divergence and the use of short wavelength, a lateral resolution better than 400 nm can be achieved without using any optical elements such as zone plates. Objects composed of different materials and thickness are used to determine the imaging properties of holographic microscopy in the VUV and soft X-ray wavelength range.2 By tuning the x-ray energy to core resonances, element specific contrast can be obtained.3 These results are promising with respect to the possibility to exploit intrinsic contrast mechanisms for biological samples. Using fibroblasts and other cells, the sensitivity of the technique to resolve small structures inside these extended objects will be discussed. Although the spatial resolution still needs to be improved, we consider these experiments as starting point for future lensless holospectroscopy and as microscopy approach for highly coherent x-ray sources such as free electron lasers.
1 D. Gabor, Nature 1948, 161, 777
2 A. Rosenhahn, R. Barth, X. Cao, M. Schürmann, M. Grunze, S. Eisebitt, Ultramicroscopy 2007, 107, 1171
3 A. Rosenhahn, R. Barth, F. Staier, T. Simpson, S. Mittler, S. Eisebitt, M. Grunze, Journal of the Optical Society of America A 2008, 25(2), 416.