Invited Paper BM+MN+BI+BO-TuM11
CD Based Sample Preparation and Pathogen Screening

Tuesday, October 21, 2008, 11:20 am, Room 309

We have demonstrated the feasibility of a multiplexed microfluidic CD apparatus for sample preparation of a wide variety of clinical samples and the subsequent detection of viruses, bacteria and fungi through fast DNA hybridization on the same platform. As the CD slowly rotates, a free moving magnetic disc in a lysis chamber is moved back and forth in the radial direction by the magnetic force of stationary magnets located below the rotating CD. The movement of the magnetic disk causes mechanical shear that disrupts cell membranes. This CD does not only process multiple samples simultaneously, but can also be used for the centrifugal precipitation of solids from each sample liquid. After precipitation, the resulting clarified liquid is transferred through a solid phase extraction membrane to capture the DNA. This step is followed by subsequent automated washing, elution, and detection by hybridization and fluorescence detection on an embedded DNA array. Recent sample to answer results and modeling of ice valves and coriolis valves will be detailed.