Paper BI-TuP7
XPS Analysis of Protein Adsorption on Bioprocessing Materials

Tuesday, October 21, 2008, 6:30 pm, Room Hall D

Protein therapeutics, a fast-growing category of biotech drugs, is adversely affected by protein aggregation. Aggregation can decrease the safety and efficacy of the therapeutic biologic protein product. Currently, the cause of protein aggregation is not clearly understood. The adsorption of protein therapeutics to bioprocessing materials used in production, storage and delivery may create nucleation sites for further protein aggregation. X-ray photoelectron spectroscopy was used to analyze protein adsorption on bioprocessing materials to screen for those with increased probability of contributing to aggregation of the protein therapeutic. The standard overlayer model and protein labeling were used to estimate the thickness of adsorbed protein layers.