| AVS 55th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session with Focus on Cells and Proteins at Interfaces |
| Presenter: | A. Hucknall, Duke University |
| Authors: | M.S. Johannes, Duke University A. Garcia, Duke University A. Hucknall, Duke University A.J. Simnick, Duke University R.L. Clark, Duke University S. Zauscher, Duke University A. Chilkoti, Duke University |
| Correspondent: | Click to Email |
We report the use of bulk microfabrication processes to pattern non-fouling, surface-initiated poly-oligoethylene glycol methacrylate (pOEGMA) polymer brushes. Previous studies have demonstrated the non-fouling capability of pOEGMA surfaces through protein absorption and cell adhesion studies. Here, we demonstrate that the polymer brushes on a glass substrate can be easily patterned using two processes. In the first one, standard photolithography is used to pattern both negative and positive photoresist spin-coated on the pOEGMA layers. The patterned photoresist layers act as masks for the removal of exposed underlying pOEGMA layers by UV-O3 exposure and oxygen plasma ashing. Upon photoresist stripping, patterns with a resolution down to ~400 nm are achieved. In the second approach, TEM grids are directly placed on the pOEGMA layer to block the brushes from direct exposure to various oxidative species offering great simplicity, high-throughput, and low-cost. Successful patterning of non-fouling surfaces at both the micron and nanometer scale will have importance in developing patterned cell monolayers and studying linear motor protein locomotion.