| AVS 55th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session with Focus on Cells and Proteins at Interfaces |
| Presenter: | H. Tatematsu, Nagoya University, Japan |
| Authors: | H. Tatematsu, Nagoya University, Japan T. Fujima, Musashi Institute of Technology, Japan N. Saito, Nagoya University, Japan O. Takai, Nagoya University, Japan |
| Correspondent: | Click to Email |
Polymer chains grafted in the high density stretch perpendicularly by repulsive interaction. This is a so-called brush. The various functional groups can be also introduced into polymer brush as lateral chains. The 3-dimensional structure was varied by the type of lateral chains. On spherical polyelectrolyte brush (PEB), strong adsorption of protein takes place at low ionic strength whereas less protein is adsorbed at the high ionic strength.1 In addition, protein structure and character remain via adsorption.2 These properties can be applied to improvement of biocompatibility and Drug Delivery System (DDS). However, the kinetics of adsorption on normal PEB surface has not been understood well. Thus, in this study, we aimed to investigate the kinetics of protein adsorption on PEB. Polystyrene brush (PSB) surface was fabricated by reacting polystyrene terminated by a trichlorosilane (PS-Cl3) group with silanol (Si-OH) groups on the silicon wafer. PSB brush was converted to polystyrene sulfonate sodium salt brush (PSSB) by sulfonation and neutralization. In protein adsorption experiment, the solution of Bovine Serum Albumine (BSA) in phosphate buffer saline (PBS) was used. NaCl aqueous solution adjusted to various concentrations was added to the stock solution in order to extract the effect of ionic strength. Protein adsorption was evaluated by Fourier transform infrared spectroscopy (FT-IR) and the evanescent adsorption spectroscopy (EOS). The proteins on the surfaces were observed by atomic force microscope (AFM). Relationship between the amount of adsorption and the ion strength on normal PSSB surface differs from that on spherical PSSB surface in previous reports. This would originate from structural configuration of PEB. Finally, we propose a kinetic model of the adsorption on PSSB based on the variation of adsorption against time and ion strength by FT-IR, EOS and AFM.
1 A. Wittemann and M. Ballauff, Phys. Chem. Chem. Phys., 2006, 8, 5269-5275
2 B. Haupt, Th. Neumann, A. Wittemann, and M. Ballauff, Biomacromolecules 2005, 6, 948-955