| AVS 55th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuM |
| Session: | Protein and Cell Interactions at Interfaces |
| Presenter: | L. Meagher, CSIRO Molecular and Health Technologies, Australia |
| Authors: | L. Meagher, CSIRO Molecular and Health Technologies, Australia H. Thissen, CSIRO Molecular and Health Technologies, Australia P. Pasic, CSIRO Molecular and Health Technologies, Australia R.A. Evans, CSIRO Molecular and Health Technologies, Australia S. Pereira, CSIRO Molecular and Health Technologies, Australia G. Johnson, CSIRO Molecular and Health Technologies, Australia G. McFarland, CSIRO Molecular and Health Technologies, Australia K. Tsang, CSIRO Molecular and Health Technologies, Australia T. Gengenbach, CSIRO Molecular and Health Technologies, Australia K. McLean, CSIRO Molecular and Health Technologies, Australia |
| Correspondent: | Click to Email |
Interest in surface initiated polymerization (SIP) to generate surface coatings for application in biomaterials has increased rapidly in the last few years, particularly those coatings generated using “living” free radical polymerization since they result in coatings with very well defined properties and architectures.1 Biomaterials applications have largely been in the control of cell attachment, achieved by varying the type of monomer used (e.g. neutral, hydrophilic coatings can be used to reduce protein adsorption and cell attachment). In this study, we present an approach to preparing such coatings which relies on the covalent attachment of a polymer containing controlled free radical initiators onto amine surfaces.2 This approach allows for maximum flexibility with respect to the substrate (unlike those using self assembled monolayers). Whilst earlier studies have relied on passive coatings, we have included biological signals by building activated functional groups into the coatings as well as drugs and molecules for the specific binding of proteins. These coatings therefore have the potential to interact with biological systems in a much more dynamic manner. XPS and colloid probe AFM were used for coating characterisation. Cell culture studies (24 hours) were carried out with either HeLa, bovine corneal epithelial or L929 fibroblast cells using standard techniques. Protein adsorption was quantified using time resolved fluorescence and proteins labeled with an Eu chelate. Examples presented in this study are the use of iniferters, ATRP initiators and RAFT agents to form SIP coatings with well defined properties. For example, dense polymer brushes which have low or high cell attachment depending on the type and amount of protein adsorption. In addition, the incorporation of cell attachment peptides into coatings via reaction with activated NHS esters will be discussed. Furthermore, the use of click chemistry to conjugate biological signals into coatings formed using our approach will be presented as well as the incorporation of molecules which allow for specific protein binding (e.g. polymerisable biotin) will be discussed in the form of application to ELISAs.
1 Edmond, S., Osborne, V.L. and Huck, W.T.S. Chemical Society Reviews, 33, 14, 2004.
2 Meagher, L., Thissen, H., Pasic, P., Evans, R.A., Johnson, G., Polymeric coatings and methods for forming them, WO2008019450-A1, 21 Feb 2008.