| AVS 55th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI+NC-TuA |
| Session: | Protein and Cells Interactions on Micro- and Nanofabricated Substrates |
| Presenter: | T.P. Beebe, Jr., University of Delaware |
| Authors: | W.M. Theilacker, University of Delaware A.L. Styer, University of Delaware D.E. Willis, Alfred I. DuPont Hospital for Children J.L. Twiss, Alfred I. DuPont Hospital for Children M.E. Boggs, University of Delaware S.P. Sullivan, University of Delaware Z. Zhang, Spansion, Inc. T.P. Beebe, Jr., University of Delaware |
| Correspondent: | Click to Email |
We have developed a method to control the local surface density of peptides and proteins that are covalently attached to various test substrates, for cell culture assays ranging from cell attachment propensity, to cell attachment density, to cellular behavior and signaling, to cell-cell interactions, for a variety of cell types and for a variety of proteins and peptides. This degree of control has recently been extended to step and continuous gradients in local protein and peptide concentrations from the micron to the centimeter length scale. Our use of these test substrates has focused mainly on neuronal cell types, for the development of new biomaterial bridging applications in brain and spinal cord injury patients, and on cell-cell interactions between osteocytes and neurons, for an understanding of “bone pain” in cancer patients. This presentation will focus on the production of these test substrates, their characterization by a variety of surface analytical and optical microscopy techniques, including XPS, TOF-SIMS, AFM, and epi-fluorescence microscopy with immunostaining, and the results of cell culture studies using these test substrates.