| AVS 54th International Symposium | |
| Plasmonics Topical Conference | Tuesday Sessions |
| Session PL-TuP |
| Session: | Plasmonics Poster Session |
| Presenter: | P. Lisboa, European Commission, Italy |
| Authors: | P. Lisboa, European Commission, Italy M.B. Villiers, INSERM U823, Institut Albert Bonniot Grenoble, France C. Brakha, INSERM U823, Institut Albert Bonniot Grenoble, France S. Cortes, INSERM U823, Institut Albert Bonniot Grenoble, France A. Valsesia, European Commission, Italy P. Colpo, European Commission, Italy P. Marche, INSERM U823, Institut Albert Bonniot Grenoble, France F. Rossi, European Commission, Italy |
| Correspondent: | Click to Email |
The interaction between biomolecules and materials is regulated by the chemical properties of the surfaces and by their micro and nano-morphologies. An accurate control of chemical contrasted motives is highly required for the improvement of the sensitivity and the specificity of biosensing devices.1,2 Polypyrrole (PPy) is a preferential material for bio-analytical sensors thanks to its good environmental stability and excellent biocompatibility, together with the possibility of being functionalised with biological relevant functional groups.@foontote 3@ In this work the gold surface of Surface Plasmon Resonance (SPR) chip was nanostructured to create gold nano-areas over a SiOx matrix by using colloidal lithography. Then, micro spots of modified PPy with ovalbumin (recognition element) and with casein (control) have been created on the nano-structured surfaces and non nanostructured surfaces by electrochemical copolymerization using a microarrayer in potentiostatic conditions. The growth of the modified PPy in the nanostructures takes place only in the gold nano-areas since the surrounded matrix of SiOx presents electrically insulating properties. The effect of the nano-structured PPy surface on the immunoreaction efficiency was evaluated using SPR imaging device. The SPR signals related to the recognition of ovalbumin by different concentrations of anti-Ovalbumin have been measured. The results were compared with a chip with micro spots of uniformly (non nanostructured) modified PPy. Even if the active area of PPy containing Ovalbumin represents only 3% of the area of non nanostructured PPy, the absolute SPR signals resultant from the ovalbumin/anti-ovalbumin reaction was higher by only a factor of 2 in the case of the uniform PPy than in the case of nanostructured surfaces. Nevertheless at lower concentrations, the SPR signal is similar showing that the nanostructured surface improves the biorecognition efficiency at these concentrations. Furthermore, the SPR signals normalised to the active PPy area were higher by a factor of 14 in the nanostructure surfaces for all the antibody concentrations. These results indicate that the nanostructured surfaces improve the immunoreaction efficiency. This is a very promising result regarding the increase of detection sensitivity in analytical devices.
1K. Lee. et al Nano-Letters, 2004 4, 1869.,
2A. Valsesia, et al, Langmuir 2006, 22, 1763.,
3L. Grosjean et al., Analytical Biochemistry 2005, 347, 193-200.