| AVS 54th International Symposium | |
| Nanometer-scale Science and Technology | Wednesday Sessions |
| Session NS1+BI-WeA |
| Session: | Biological and Molecular Applications of Nanoscale Structures |
| Presenter: | P. Colpo, European Commission - Joint Research Centre, Italy |
| Authors: | A. Valsesia, European Commission - Joint Research Centre, Italy P. Colpo, European Commission - Joint Research Centre, Italy I. Mannelli, European Commission - Joint Research Centre, Italy P. Lisboa, European Commission - Joint Research Centre, Italy F. Bretagnol, European Commission - Joint Research Centre, Italy G. Ceccone, European Commission - Joint Research Centre, Italy F. Rossi, European Commission - Joint Research Centre, Italy |
| Correspondent: | Click to Email |
The next challenge for the development of analytical devices for biological analysis relies on the ability to design advanced surfaces able to interact properly with the biological world. An increase of several order of magnitude of analysis capacity in biosensing devices together with lower detection limits is envisaged, due to the special interactions between the biomolecules and the nanostructured materials. The most important consequences of the nano-structuring of the bio-interacting surfaces is the immobilization of the biomolecular probes in an active state, limiting the non specific adsorption and the optimisation of their binding site accessibility for the bio-recognition of the target molecules. In our laboratory, we have developed alternative fabrication strategies for the creation of chemically nanostructured surfaces by combining Colloidal Lithography and Electron Beam Lithography with Surface Functionalization Techniques such as Plasma Enhanced Chemical Vapour Deposition (PE-CVD) of bio-functional polymers and Self Assembled Molecular Monolayers (SAM). In particular carboxylic functionalized nano-domes in a PEO-like anti-fouling matrix have been produced. We showed that these chemical nano-patterns are able to immobilize proteins selectively in the carboxylic functional nano-domains, leaving the anti-fouling matrix clear Moreover, we have compared the detection performances between uniformly functionalized surface and chemically nano-patterned surfaces when applied as platforms for antigen/antibody interactions. In particular, homogeneous PAA was compared with the PAA nano-areas in anti-fouling matrix previously described. Nano-patterned surfaces showed a considerable enhancement of the immunoreaction efficiency with respect to the non-structured surfaces, demonstrating the capability of nano-patterns to improve the binding site accessibility of the immobilized biological probes.