| AVS 54th International Symposium | |
| Nanometer-scale Science and Technology | Wednesday Sessions |
| Session NS1+BI-WeA |
| Session: | Biological and Molecular Applications of Nanoscale Structures |
| Presenter: | N.A. Amro, NanoInk, Inc. |
| Authors: | N.A. Amro, NanoInk, Inc. S. Rozhok, NanoInk, Inc. T. Renner, NanoInk, Inc. J. Fragala, NanoInk, Inc. M. Nelson, NanoInk, Inc. |
| Correspondent: | Click to Email |
Miniaturized nucleic acid in the form of nanoarrays will dramatically enhance the sensitivity, and spatial density of chip-based bio-assays. These nanoarrays will improve applications ranging from point-of-care diagnosis to genomic arrays used in basic research by enabling the development of next generation screening technologies that are faster, more sensitive, more reliable, and possibly more cost effective than those presently available in the life sciences market. Nucleic acid bioarrays can be printed using Dip-Pen Nanolithography® (DPN®), a new direct-write spotting technology which generates sub-micron sized features of DNA or protein on solid surfaces. This printing technique offers significant advantages over current microarray printing technologies that suffer from poor spot to spot reproducibility in terms of size, shape, and oligonucleotides density, as well as reproducibility across microarray slides. In this talk we report on recent technical advances in patterning DNA and protein patches with submicron dimensions on glass and metal coated substrates, using new commercially available DPN accessories such as multiple pen arrays and microfluidic pen loading which allows performing multiplexed DNA and protein patterning to fabricate arrays with nanoscale registration and high throughput printing. DNA and protein dot features as small as 200 nm in diameter can be routinely generated. DPN patterning of proteins and oligos, as well as screening for their biological activity, will be shown and discussed in detail.