| AVS 54th International Symposium | |
| Nanometer-scale Science and Technology | Tuesday Sessions |
| Session NS-TuP |
| Session: | Nanometer-scale Science and Technology Poster Session I |
| Presenter: | A. Gunnarsson, Lund University, Sweden |
| Authors: | A. Gunnarsson, Lund University, Sweden P. Jönsson, Lund University, Sweden R. Marie, Lund University, Sweden J. Tegenfeldt, Lund University, Sweden F. Höök, Lund University, Sweden |
| Correspondent: | Click to Email |
Development of ultrasensitive biological sensors has attracted significant scientific interest due to its many possible application areas such as biomedical diagnostics, proteomics and forensic analysis. DNA sensing using nanoscale devices based on electrical1,2 or optical3,4 detection has shown great promise for achieving high sensitivity and specificity. We report of a novel biosensor assay capable of detecting non-labeled low-abundant DNA targets based on surface adsorption of lipid vesicles through DNA hybridization. Using total internal reflection fluorescence (TIRF) microscopy, single DNA molecule detection at 10 fM concentrations is achieved. Various biomolecules besides DNA can be probed with the assay, including soluble and membrane proteins utilizing the lipid bilayer of the vesicles. Not only achieving ultra-sensitive detection, the novel approach also offers great potential for studies of biomolecular interactions on a single molecule level in real time.
1 Hahm, J.; Lieber, C. M. Nano Letters 2004, 4, (1), 51-54.
2 Brousseau, L. C. Journal of the American Chemical Society 2006, 128, (35), 11346-11347.
3 Bailey, R. C.; Nam, J. M.; Mirkin, C. A.; Hupp, J. T. Journal of the American Chemical Society 2003, 125, (44), 13541-13547.
4 Ho, Y. P.; Kung, M. C.; Yang, S.; Wang, T. H. Nano Letters 2005, 5, (9), 1693-1697.