| AVS 54th International Symposium | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session |
| Presenter: | N. Saito, Nagoya University, Japan |
| Authors: | N. Saito, Nagoya University, Japan Y. Sato, Nagoya University, Japan O. Takai, Nagoya University, Japan |
| Correspondent: | Click to Email |
The control of blood coagulation on biomaterial surfaces is an urgent issue in the medical field. The main process occurring in blood coagulation is the polymerization of fibrinogen into erythrocyte-trapping fibrin. Other plasma proteins are also involved in initiating this process. The multi-interactions among proteins and material surface govern the process. The molecular structure of fibrinogen as a free molecule has been investigated in detail. However, its adsorption state on a biomaterial surface has yet to be elucidated. Recently, the adsorption state of fibrinogen on various substrates has been the subject of intensive investigation employing atomic force microscopy (AFM). Such use of AFM has been crucial in the study of molecular biology on surfaces. In this study, the adsorption kinetics and dynamics of fibrinogen with the interaction of heparin onto hydrophobic and hydrophilic surfaces is investigated with AFM analysis and QCM (quartz crystal microbalance). CH3-terminated self-assembled monolayer (SAM) and SiOH-terminated substrates were utilized as the hydrophobic and hydrophilic surfaces. Fibrinogen adsorption proceeded following Langmuir type. On the process, the orientation of fibrinogen on the sample surface was changed. Heparin did not promote desorption of fibrinogen but the adsorption on the hydrophilic surface though it is well known as an anti-coagulation factor. On the other hands, heparin promoted the adsorption on the hydrophobic surface. The differences were discussed based on 3-body interactions analysis.