AVS 54th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP26
"Smart" Biopolymer for Reversible Stimuli-Responsive Platform of Cell-Based Biochips

Tuesday, October 16, 2007, 6:00 pm, Room 4C

Session: Biomaterials Interfaces Poster Session
Presenter: K. Na, Seoul National University, Korea
Authors: K. Na, Seoul National University, Korea
O. Kim, Seoul National University, Korea
J. Jung, Seoul National University, Korea
J. Lee, Seoul National University, Korea
J.W. Park, Korea Research Institute of Standards and Science
T.G. Lee, Korea Research Institute of Standards and Science
J. Hyun, Seoul National University, Korea
Correspondent: Click to Email

In the presentation, we describe the genetical synthesis of lysine tagged ELP (ELP-K) with inverse phase transition temperature (Tt) of 30? for fabricating a thermo-responsive culture surface. For the micropatterning of ELP-K a removable polymer template was microcontact-printed on the glass surface derivatized with epoxide. After conjugating ELP-K onto the glass surface followed by dissolving a polymer template, highly resolved ELP-K micropatterns were efficiently created on the surface. The successful micropatterning of the polypeptide was confirmed using ELP-K conjugated with fluorescence dye by confocal microscopy as well as atomic force microscopic images. TOF-SIMS images of ELP-K micropatterns verified the highly resolved ELP microstructure on the surface.