AVS 54th International Symposium | |
Biomaterial Interfaces | Tuesday Sessions |
Session BI-TuP |
Session: | Biomaterials Interfaces Poster Session |
Presenter: | K. Na, Seoul National University, Korea |
Authors: | K. Na, Seoul National University, Korea O. Kim, Seoul National University, Korea J. Jung, Seoul National University, Korea J. Lee, Seoul National University, Korea J.W. Park, Korea Research Institute of Standards and Science T.G. Lee, Korea Research Institute of Standards and Science J. Hyun, Seoul National University, Korea |
Correspondent: | Click to Email |
In the presentation, we describe the genetical synthesis of lysine tagged ELP (ELP-K) with inverse phase transition temperature (Tt) of 30? for fabricating a thermo-responsive culture surface. For the micropatterning of ELP-K a removable polymer template was microcontact-printed on the glass surface derivatized with epoxide. After conjugating ELP-K onto the glass surface followed by dissolving a polymer template, highly resolved ELP-K micropatterns were efficiently created on the surface. The successful micropatterning of the polypeptide was confirmed using ELP-K conjugated with fluorescence dye by confocal microscopy as well as atomic force microscopic images. TOF-SIMS images of ELP-K micropatterns verified the highly resolved ELP microstructure on the surface.