| AVS 54th International Symposium | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session |
| Presenter: | O. Ramstrom, Royal Institute of Technology, Sweden |
| Authors: | O. Ramstrom, Royal Institute of Technology, Sweden M. Yan, Portland State University |
| Correspondent: | Click to Email |
Photogenerated bioactive surfaces have been developed following a perfluorophenylazide-based, double ligation strategy. Gold-plated quartz crystal microbalance crystals were initially coated with different polymers, either covalently or non-covalently, and the resulting surfaces were subsequently used as substrates for photoinsertion of active photoprobes. A range of different polymers were evaluated, where high biocompatibility and durability could be shown. The photoprobe insertion yielded a range of different carbohydrate-based recognition motifs presented at the surfaces, which were subsequently used as sensor recognition elements in a flow-through quartz crystal microbalance instrumentation. A series of different carbohydrate-binding proteins (lectins) were analyzed, and selectivity and affinity of protein binding could be efficiently monitored. The results clearly show the predicted protein selectivities, demonstrating the applicability of the approach.