| AVS 54th International Symposium | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI-ThP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | J.S. Shumaker-Parry, University of Utah |
| Authors: | J. Liu, University of Utah M.A. Eddings, University of Utah B.K. Gale, University of Utah J.S. Shumaker-Parry, University of Utah |
| Correspondent: | Click to Email |
Surface plasmon resonance (SPR) microscopy provides quantitative, real-time information about adsorption and desorption on an SPR-active sensor surface with high spatial resolution. Label-free, high-throughput analysis of biomolecule interactions is made possible by combining patterned biomolecule immobilization with SPR microscopy. Typically, the biomolecules are immobilized ex situ using a pin-based microspotting device with many parameters that must be well-controlled in order to create an active and reliable sensor surface. We demonstrate the combination of a high-throughput microfluidic device with SPR microscopy for quantitative, in situ antibody immobilization. The microfluidic device provides 48 separate flow channels that can be used simultaneously for antibody immobilization and subsequent antibody-antigen interaction analysis. Because the biomolecules can be immobilized in situ, exposure to harsh environments can be avoided, a major benefit for protein immobilization. In addition, the biomolecule immobilization process can be monitored in real time by SPR microscopy and characterized quantitatively. Applications in immunoassay development for studying patient immunogenic response to antibody-based drugs will be described.