| AVS 54th International Symposium | |
| Applied Surface Science | Thursday Sessions |
| Session AS-ThP |
| Session: | Aspects of Applied Surface Science II Poster Session |
| Presenter: | V. Altuzar, Universidad Veracruzana, Mexico |
| Authors: | A. Diaz-Badillo, CINVESTAV-IPN, Mexico V. Altuzar, Universidad Veracruzana, Mexico J.G. Mendoza-Alvarez, CINVESTAV-IPN, Mexico A. Cisneros, Universidad Autonoma Benito Juarez, Mexico F. Jimenez-Rojas, Salud Pública y Servicios de Salud de Oaxaca, Mexico J.P. Martinez-Muñoz, Salud Pública y Servicios de Salud de Oaxaca, Mexico J.L. Herrera-Perez, CICATA-IPN, Mexico C.O. Mendoza-Barrera, Universidad Veracruzana, Mexico F. Sanchez-Sinencio, CINVESTAV-IPN, Mexico P. Gariglio-Vidal, CINVESTAV-IPN, Mexico M.L. Muñoz, CINVESTAV-IPN, Mexico |
| Correspondent: | Click to Email |
Dengue is a mosquito-borne viral infection causing a major public health problem globally. Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic fever and includes four distinct serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). DEN-2 and DEN-3 have been associated with severe dengue disease. In this work we demonstrated a high throughput of microarrays for detection of dengue virus in serum samples from patients with a defined dengue infection from Oaxaca or in mosquito population collected across eighteen Mexican states. We have applied microarray analysis for simultaneous serotyping multiple RNA samples from human or mosquitoes through the NS3 genome. The proposed microarray method can be used for i) rapid and reliable dengue diagnosis; ii) serotyping and iii) surveillance of mosquitoes infected with dengue. Moreover by using these microarrays we have determined DEN viruses in pools of gravid females mosquitoes collected in several sites of eighteen Mexican states in 2005. Our microarrays were also useful to confirm the presence of DEN-2 in 96 serum samples, DEN-3 in three samples from Oaxaca city and one case from Juchitán, Oaxaca contained DEN-2 and -3. The microarrays quantification were validated by using RT-PCR, in fact they presented agreement in all cases excepting with those mosquito samples collected in one site of Chiapas, Oaxaca, Morelos and Nayarit. In conclusion, we show the success of applying microarrays assay to provide a consistently robust qualitative detection of dengue serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) in serum samples from patients or in pools of gravid female mosquitoes collected in the field of eighteen Mexican states.