AVS 51st International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP32
Deposition of Lipid Bilayers on the Silicon Dioxide Surfaces Patterned by Focused Ion Beam and Synchrotron Radiation Etching

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: R. Tero, Institute for Molecular Science, Japan
Authors: R. Tero, Institute for Molecular Science, Japan
M. Rahman, The Graduate University for Advanced Studies, Japan
Z.-H. Wang, Nagoya University, Japan
M. Sugawara, Nihon University, Japan
K. Nagayama, National Institute for Physiological Sciences, Japan
T. Urisu, Institute for Molecular Science, Japan
Correspondent: Click to Email
Nano-bioelectronics is one of the most attractive research fields in these days. Microfabrication and modification with biomaterials on solid surfaces have fascinated enormous attentions as important techniques for the development of new biosensors and new devices. We have fabricated fine structures on SiO@sub 2@/Si surfaces by combination of the focused ion beam (FIB) and the synchrotron radiation (SR) etching, and deposited lipid bilayers on the SiO@sub 2@ surface by the vesicle fusion method. FIB patterning was performed using JEOL-JFIB2300 with the 30 keV of Ga ion. SR etching was performed in the BL4A2 in UVSOR in IMS using Co photomask with the thickness of 400 nm under the mixture gas of SF@sub 6@ and O@sub 2@ (P@sub SF6@=5.0x10@super -2@ Torr, P@sub O2@=2.0x10@super -3@ Torr). AFM images were obtained by Picoscan (Molecular Imaging). In the deposition of lipid bilayers, the sample was incubated above the gel-liquid crystal transition temperatures in the suspension of the pure dipalmitoylphosphatidylcholine (DPPC) or the mixture of egg PC, dioleoylphosphatidylethanolamine (DOPE) and cholesterol (3:1:1), which were prepared by agitating the vacuum-dried lipid films in a buffer solution. We have patterned the Co photomask on the SiO@sub 2@/Si substrate by FIB in micrometer order. Three-dimensional micrometer-order structures were successfully obtained by one time SR irradiation (2.0x10@super 4@ mA min). After deposition of DPPC vesicles on the SiO@sub 2@ surface, flat membranes with the height of 5 nm were observed by atomic force microscopy (AFM). The thickness of the membranes well corresponded to that of the single lipid bilayer. The morphology, electronic resistance and additional effect of protein (gramicidin A) will be discussed.