AVS 51st International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP28
Compartmentalized Bioreactor for Long-Term Culture of Bone Cells

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: R. Dhurjati, Pennsylvania State University
Authors: R. Dhurjati, Pennsylvania State University
E.A. Vogler, Pennsylvania State University
P.W. Brown, Pennsylvania State University
H.J. Donahue, Pennsylvania State University
Correspondent: Click to Email
A compartmentalized bioreactor designed around the concept of continuous growth and dialysis, was used to study long-term (15-30 days) phenotypic behavior of human fetal osteoblastic cells (hFOB). This specific design separates the growth and feeding functions and permits long term culture sustaining the pericellular environment, otherwise disturbed by continuous or punctuated growth medium replacement. The attachment and growth behavior of model human osteoblasts cultured in the bioreactor was evaluated using scanning and transmission electron microscopy (SEM/TEM) and were compared to those obtained from conventional tissue culture methods. Results suggest that stable culture conditions afforded by the reactor has enhanced utility in the long-term culture of osteoblasts in terms of growth, proliferation and mineralized matrix deposition characteristics and would serve as an ideal in-vitro test system for the study of cell/protein mediated interactions with synthetic bone analog materials.