AVS 51st International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP26
Detergency Effectiveness with Respect to Proteins

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: M. Richard, Ecole Centrale de Lyon, France
Authors: M. Richard, Ecole Centrale de Lyon, France
T. Le Mogne, Ecole Centrale de Lyon, France
J. Criquelion, Laboratoires Anios, France
A. Perret-Liaudet, Hopital Neurologique de Lyon, France
J.M. Martin, Ecole Centrale de Lyon, France
Correspondent: Click to Email
In the detergency field of re-usable medical devices, a special attention is focused on the non-conventional transmissible agent called prions which is a proteinaceous infectious agent. Few cleaning procedures are effective against prions and few techniques are available to study cleaning effectiveness with respect to proteins in general. The first part of our study shows that X-ray Photoelectron Spectroscopy (XPS) is a useful and reliable technique to evaluate detergent formulations effectiveness to remove proteins from stainless steel surface soiled with a human brain homogenate. A semi-quantitative evaluation of the detergency effectiveness could also be performed. XPS makes it possible to study chemical species remaining on surface, substrate properties after cleaning procedures and also the water quality effect on detergency effectiveness. In the second part of our study and in the light of the complexity of the previous system, a simplification of each part of the system was carried out. XPS analysis was used to study the effectiveness of some simple chemical functions to remove proteins from a native oxide layer of a pure chromium surface. The results will be presented and discussed in this paper.