AVS 51st International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP18
Plasma Grafted Anti-Fouling Films on Ethylene Oxide Base for Biosensors and Biotechnologies

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: M. Kormunda, EU-JRC-IHCP, Italy
Authors: M. Kormunda, EU-JRC-IHCP, Italy
G. Ceccone, EU-JRC-IHCP, Italy
A. Papadopoulou, EU-JRC-IHCP, Italy
M. Hasiwa, EU-JRC-IHCP, Italy
F. Rossi, EU-JRC-IHCP, Italy
Correspondent: Click to Email
The generation of anti-fouling surface is a key element in the design of biosensors, medical devices and implants. Protein adsorption resistant surfaces have to avoid or reduce non-specific protein adsorption, platelet adhesion and thrombus formation, to prevent undesirable responses of the living systems to the device. The highly cross-linked anti-fouling coatings on ethylene oxide base were plasma grafted from Dimethoxydiethylene glycol vapour in mixture with reactive (N@sub 2@) or inert (Ar) gas at low-pressure 40mTorr. The plasma discharge in capacitive configuration of reactor was powered by continuous (from 10 to 100W) or pulsed RF (13.56MHz) voltage. The coatings were plasma grafted on glass, Si and PTFE substrates placed on grounded substrate holder. The film chemical composition has been analysed by XPS and FTIR as well as the surface morphology (AFM) and the protein adsorption (QCM-D). The coatings toxicity and an adhesion of Fibroblast L929 have been investigated on selected samples. The lower power plasma grafted coatings have very good antifouling properties together with low receding contact angles although the methyl function groups in the films are more populated than ethylene oxides groups. The coatings are stable with low contact angles over period about 6 months at normal conditions. No significant changes were observed on functional groups in coatings washed for 40 days in ethanol bath. The oxygen plasma etching during a nano-patterning process for biosensors fabrication has not significant influence on chemical composition, surface morphology and contact angles.}