AVS 51st International Symposium
    Biomaterial Interfaces Thursday Sessions
       Session BI+AS+SE-ThM

Paper BI+AS+SE-ThM11
Spectroscopic Characterization of Surface-Immobilized Antibacterial Furanone Coatings

Thursday, November 18, 2004, 11:40 am, Room 210D

Session: Surface Modification of Biomaterials
Presenter: S. Al-Bataineh, University of South Australia
Authors: S. Al-Bataineh, University of South Australia
H.J. Griesser, University of South Australia
M. Willcox, University of New South Wales, Australia
L.G. Britcher, University of South Australia
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The colonisation by bacteria of biomedical devices presents a serious concern for human implant surgery. In this study, we explore how bacterial colonisation can be prevented by the appropriate design and fabrication of antibacterial coatings, with a major focus on surface-immobilised furanone molecules. These compounds are produced naturally by the marine algae, Delisea pulchra and are used as defence agents to prevent fouling on their surface@footnote 1@. Several studies have shown that brominated furanones as well as synthetic analogues possess potent antimicrobial activity against bacteria@footnote 2@@super ,@@footnote 3@. The previously used azide protocol was adopted to prepare furanone coatings@footnote 4@. XPS and ToF-SIMS results showed successful surface modifications and furanone immobilisation. Detailed analysis of the C 1s and N 1s XPS spectra using constrained curve fitting showed that they are more complicated than anticipated from the theoretical reaction scheme. In addition, the presence of a Br@super-@ peak partially overlapped with a C-Br peak indicated that furanones are partially degraded on UV illumination. More surface characterisations are needed for full understanding of the chemical reactions that occurred. Seven furanone compounds used in this study were tested for their ability to inhibit biofilm formation and growth of two bacterial strains, Staphylococcus aureus (Saur19) and Pseudomonas aeruginosa (Paur6206). Initial results are promising; detailed investigation of the efficacy of the coatings is ongoing. Furthermore, none of the compounds used in this study showed any cytotoxicity potential at the tested concentrations. @FootnoteText@ @footnote 1@ de Nys R. et al., 1995, 4:259-71.@footnote 2@ Kjelleberg S. et al., Patent No. PCT/AU99/00284, 1999.@footnote 3@ Read R. et al. PCT international application PQ6812, 2001.@footnote 4@ Muir B. et al., Proc. 6th World Biomat. Congr., Hawaii, May 2000, p. 596.