AVS 51st International Symposium
    Applied Surface Science Monday Sessions
       Session AS-MoP

Paper AS-MoP3
Cluster Primary Ion Bombardment Facilitates ToF-SIMS Analysis of Biological/Tissue samples

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: V.S. Smentkowski, General Electric Global Research Center
Authors: V.S. Smentkowski, General Electric Global Research Center
A. Schnieders, Ion-Tof USA, Inc.
F. Kollmer, Ion-Tof GmbH
R. Kersting, Tascon GmbH, Germany
J.A. Ohlhausen, Sandia National Laboratories
M.R. Keenan, Sandia National Laboratories
P.G. Kotula, Sandia National Laboratories
Correspondent: Click to Email
Biological/tissue samples present a number of analytical challenges including the detection of trace quantities of high mass species within large (few mm) areas of samples. Recent advances in both ToF-SIMS instruments (such as stage rastering and liquid metal polyatomic ion sources) and data reduction protocol are facilitating biological/tissue analysis. In this poster, we will compare ToF-SIMS spectra and images collected using both Ga (mono-atomic) and Gold 3 (polyatomic) primary ion sources on treated brain tissue sections. Using polyatomic gold we are able to detect and image high mass (> 1,500 amu) species; these species were not detected in the spectra collected using Ga (even with a higher Ga primary ion fluence). The wealth of information contained in spectral images collected using polyatomic gold is tremendous, and the resulting spectral images can be difficult to interpret. In this poster we will also demonstrate that Multivariate Statistical Analysis (MVSA) tools such as AXSIA (Automated eXpert Spectrum Image Analysis) are useful for deconvoluting the spectral images. Using AXSIA, we are able to separate low yield species from high yield species in the high mass (ca. 760 amu) region of the ToF-SIMS spectra. The AXSIA component of this work was funded in part under CRADA SC00/01609 PTS 1609.02 (GE/Sandia). Sandia is a multiprogram laboratory operated by Sandia Corporation, a Lockheed Martin Company, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.