AVS 51st International Symposium
    Applied Surface Science Monday Sessions
       Session AS-MoP

Paper AS-MoP1
Producing Substrates for MALDI-MS by Chemomechanically Scribing Silicon and Glass.

Monday, November 15, 2004, 5:00 pm, Room Exhibit Hall B

Session: Poster Session
Presenter: R. Blake, Brigham Young University
Authors: R. Blake, Brigham Young University
G. Jiang, Brigham Young University
C. Thulin, Brigham Young University
M.R. Linford, Brigham Young University
Correspondent: Click to Email
Miniaturized matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sample supports have been developed on silicon and glass for improving analysis sensitivity and reproducibility of biomolecules. Clean silicon and glass were silanized with a fluorinated compound that produces extremely hydrophobic surfaces. After silanization 200 micron diameter hydrophilic anchors were chemomechanically scribed with a diamond tip onto silicon and glass in the presence of water. These hydrophilic areas pin aqueous solutions of matrix and peptide. As the water evaporates the matrix and peptide are pinned to the hydrophilic areas, concentrating and localizing the analyte to a small spot that is fully covered by the ionizing laser beam. Thus, both sensitivity and reproducibility of the analysis are simultaneously improved. MALDI-MS spectra of water soluble peptides, glufibrinopeptide B and angiotensin II, with 2,5-dihydroxy benzoic acid (DHB) as matrix on silicon or glass substrates showed the expected improvements both in sensitivity and reproducibility. Compared to previous reports, this method of making miniaturized MALDI-MS sample supports is rapid, cheap, and could be undertaken in a typical laboratory.