AVS 51st International Symposium
    Applied Surface Science Wednesday Sessions
       Session AS+BI-WeA

Paper AS+BI-WeA8
Characterization of Bound Nucleotides using XPS and ATR-FTIR

Wednesday, November 17, 2004, 4:20 pm, Room 210A

Session: Biological Applications of Surface Analysis
Presenter: D.B. Barbash, The University of New Mexico
Authors: D.B. Barbash, The University of New Mexico
J.E. Fulghum, The University of New Mexico
G.P. Lopez, The University of New Mexico
Y. Wu, The University of New Mexico
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In this work we utilize XPS and ATR-FTIR to probe the attachment of nucleotides to self-assembled monolayers. The attachment of DNA onto functional surfaces is utilized in applications ranging from DNA microarray technology to molecular wires. We are utilizing nucleotides as model systems in the development of surface-specific methods for the analysis of bound DNA. The nucleotides or DNA are covalently bound to self-assembled monolayers on glass or gold substrates using a diazotization-based method we recently developed (Dolan, P.L. et al. Nucleic Acids Research 2001, 29, 21e107). Three of the four nucleotides can be attached using this method, and we report unique spectral signatures for the nucleotide bases adenine (dATP), cytosine (dCTP), and guanine (dGTP) based on ATR-FTIR and XPS analyses. Based on these spectral signatures, results of competitive binding experiments will be discussed. XPS is also utilized to characterize the attachment steps and estimate surface coverage. ARXPS results of bound nucleotides will be discussed.