Paper BI-WeM3
Cytocompatible Mineralization on Jurkat T Cell Surfaces with Titania Composites

Wednesday, December 10, 2014, 8:40 am, Room Milo

The artificial shells of organic/inorganic materials on living cells would give new properties to the encapsulated cells. For example, the encapsulate cell could live against physical deformation and chemical hazards, and control the cell division. Also, functionalization would be easier than native cell surface. Recently, our paper said that the (RKK)₄D₈ peptide had both the TiO₂-inducing and cytocompatible units for Chlorella cell. However, due to the fragile property of mammalian cells, there are few studies on the mammalian cell encapsulation.

In this work, individual Jurkat T cells were encapsulated within peptide/TiO₂ composite shells by layer-by-layer assembly and bioinspired mineralization. The cell viability and shape were maintained during the encapsulation processes, and the division of the encapsulated cells was changed by the artificial TiO₂ shell. There are cluster of differentiation 3 (CD3) antigens on the Jurkat T cell surface. After encapsulation, anti-CD3 antibody was hindered to bind CD3 antigens on the cell. For the functionalization of cell surface, TiO₂ composites made it possible to anchor the ligands of interest to the shell. After formation of the TiO₂ shells on cell surfaces, the shells were functionalized via catechol chemistry in a cytocompatible fashion. We believe that these new properties on the Jurkat T cell surface could be apply to cell-based sensors and assays as well as for fundamental studies such as immunology.