| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2014) | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | Tomohide Takami, Hiroshima University, Japan |
| Authors: | T. Takami, Hiroshima University, Japan J. Uewaki, Hiroshima University, Japan H. Ochiai, Hiroshima University, Japan M. Koyama, Tokyo University of Agriculture & Technology, Japan Y. Ogawa, Tokyo University of Agriculture & Technology, Japan M. Saito, Tokyo University of Agriculture & Technology, Japan H. Matsuoka, Tokyo University of Agriculture & Technology, Japan Y. Ojiro, Tohoku University, Japan K. Nishimoto, Tohoku University, Japan S. Ogawa, Tohoku University, Japan Y. Takakuwa, Tohoku University, Japan S. Tate, Hiroshima University, Japan |
| Correspondent: | Click to Email |
Glass nanopipettes have been used as a bridge to connect macro world and micro world.[1] They can be used as an ion-selective probe,[2-6] and as an injector to deposit a small amount of materials onto a surface.[7]
Injection to cell is a hot topic for the statistical experiments on the live dynamics of injected molecules in cell as well as the application to genetic engineering. Several auto-injection systems are already commercially available. However, the fatal problem of these auto-injection systems is the viability of cells after the injection; usually less than 10%.
We have been developing an auto-injection system in which the distance between the injecting pipette tip top and the cell is monitored and the signal depending on the pipette-cell distance is put into the feedback system to achieve the controlled insertion/extracion motion of the pipette to the cell in order to increase the viability of cells. This system is inspired from scanning tunneling microscopy on which the tip-sample distance is well-controlled for the nanoscale observation and molecular manipulation.[8]
Also, we have developed two methods for the auto-injection. One is the coating of the pipette top with chlorobenzene-terminated polysiloxane to reduce the damage to the inserted cell. The other is the gas-flow method to evaluate the inner diameter and the shank length of the pipette before using since the pipettes after the observation with electron microscope cannot be used.
In this paper, we will show our progress to realize the auto-injection system for the use of statistic and quantitative studies. We will demonstrate the ability of manual injection system to show the limit of the manual injection study. We will also demonstrate how the surface science technologies including scanning probe microscopy (SPM), surface coating, and vacuum science can be utilized for the development of the auto-injection system.
[1] T. Takami, B. H. Park, and T. Kawai, Nano Convergence 1, 17 (2014) [review paper].
[2] J.W. Son, T. Takami, J.-K. Lee, B.H. Park, and T. Kawai, Appl. Phys. Lett., 99, 033701 (2011).
[3] X.L. Deng, T. Takami, J.W. Son, T. Kawai, and B.H. Park, Appl. Phys. Express5, 027001 (2012).
[4] T. Takami, F. Iwata , K. Yamazaki , J.W. Son , J.-K. Lee , B.H. Park , and T. Kawai, J. Appl. Phys., 111, 044702 (2012).
[5] X.L. Deng, T. Takami, J.W. Son, E.J. Kang, T. Kawai, and B.H. Park, Journal of Nanoscience and Nanotechnology, 13, 5413-5419 (2013).
[6] E.J. Kang, T. Takami, X.L. Deng, J.W. Son, T. Kawai, and B.H. Park, J. Chem. Phys. B118, 5130-5134 (2014).
[7] F. Iwata, S. Nagami, Y. Sumiya and A. Sasaki, Nanotechnology18, 105301 (2007).
[8] T. Takami, e-J. Surf. Sci. Nanotech. 12, 157–164 (2014) [review paper].