| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2014) | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | Yu-Sheng Hsiao, Ming Chi University of Technology, Taiwan, Republic of China |
| Authors: | Y.-S. Hsiao, Ming Chi University of Technology, Taiwan, Republic of China H.-h. Yu, Academia Sinica, Taiwan, Republic of China H.-R. Tseng, University of California, Los Angeles P. Chen, Academia Sinica, Taiwan, Republic of China |
| Correspondent: | Click to Email |
Here we develop a universal solution-processing approach for producing three dimensional (3D) conducting polymer-based bioelectronic interfaces (BEIs), which can be integrated on chips for rare circulating tumor cell (CTC) isolation and detection. Based on the modified poly(dimethylsiloxane) (PDMS) transfer printing technology and bioconjugation process, the poly(3,4-ethylenedioxythiophene) (PEDOT)-based micro/nanorod array films can be fabricated with topographical and chemical control, respectively. This 3D PEDOT-based BEI film features the advantageous characteristics: (1) diverse dimensional structures (tunable from the microscale to the nanoscale), (2) varied surface chemical properties (tunable from nonspecific to specific), (3) high electrical conductivity, and (4) reversible electrochemical switching, and (5) high optical transparency. Furthermore, we integrated this 3D PEDOT-based BEI onchips, which exhibited optimal cell-capture efficiency from MCF7 cells was approximately 85%; featured highly efficient performance for the cell isolation of rare CTCs with minimal contamination from surrounding nontargeted cells (e.g., EpCAM-negative cells, white blood cells); preserved the cell viability with negligible effect on cells. According to the electric cell-substrate impedance sensing concept, the 3D BEI-based device was also demonstrated as a rapid, sensitive and specific tool for CTC detection. Therefore, it is conceivable that use of this platform will meet the requirements on developing for the next-generation bioelectronics for biomedical applications.
Keywords: Poly(3,4-ethylenedioxythiophene) (PEDOT), bioelectronic interfaces (BEIs), circulating tumor cell (CTC), epithelial cell adhesion molecule (EpCAM).