| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2014) | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI-MoM |
| Session: | Nanobio Imaging |
| Presenter: | Lara Gamble, University of Washington, USA |
| Authors: | L.J. Gamble, University of Washington, USA B. Bluestein, University of Washington, USA D.J. Graham, University of Washington, USA |
| Correspondent: | Click to Email |
Imaging mass spectrometry can provide images of cells and tissues with chemical and molecular specificity. These chemically specific images could revolutionize our understanding of biological processes such as increasing our understanding of chemical changes in cells and tissues as a function of an applied stress or as a result of disease, and enable tracking the spatial distribution of metabolites and lipids. The mass spectral imaging capability of ToF-SIMS holds potential to achieve this goal with sub-cellular resolution. Chemistry of tumor microenvironments, lipid metabolomics relationship to cancer, delivery of nanoparticles to cells, and tissue repair could be visualized on a cellular and sub-cellular level. In this presentation, ToF-SIMS analysis of biological samples from 2D images of tissues to 3D images of nanoparticles in cells will be presented. Challenges with sample preparation for the ToF-SIMS environment and processing of the large amount of data will be discussed (including multivariate analysis of the ToF-SIMS image data). The advantage of combining ToF-SIMS images with optical images of the same samples (same slices and serial biopsy slices) will also be presented. This combination of images allows researchers to visualize a molecular map that correlates with specific biological features or functions.