| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2014) | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI-MoE |
| Session: | Biofouling |
| Presenter: | Yu-Tzu Huang, Chung Yuan Christian University, Taiwan |
| Authors: | Y.T. Huang, Chung Yuan Christian University, Taiwan W.-J. Chen, Chung Yuan Christian University, Taiwan |
| Correspondent: | Click to Email |
The metal-modification on the surface of nanomaterials are extensively used in biomedical and environmental applications recently. Numerous noval nanocomposite materials have been developed; however, reactivity of the biological effects of these nanomaterials towards living organisms is insufficient. Here, we studied the antibacterial reactivity of two kinds of metal containing nanomaterials: (1) metal organic frameworks (MOFs): iron, chromium, aluminum and (2) hydroxyapatite with metal inclusion (gold or silver). Results of the minimum inhibitory concentration (MIC), half maximal inhibitory concentration (IC50), gene expression profile, quantitative gene expression levels, and scanning electron microscopy imaging were used to investigate the possible antibacterial mechanisms. The expressions of six genes (16S ribosomal RNA, DNA polymerase I, DNA polymerase II, cytochrome d complex, glucan biosynthesis protein G, and D-glyceraldehyde-3-phosphate dehydrogenase) indicated the genotoxicity is highly related to membrane or cell wall proteins. In addition, the toxic effects were dominant in iron/silver containing nanomaterials than chromium/aluminum/gold ones. Our findings have opened doors for understanding the insight reactivities of metal-modified nanomaterials, which will help their applications with controlled safety.